A novel Fe-Cr-B-C-Al-Si-Mn composite coating was designed and synthesized on 2Cr13 steel substrate using laser cladding technique in this study. The microstructure evolution, microhardness and wear resistance of the composite coating were investigated. The results showed that the microstructure of the coating was mainly composed of alpha-(Fe,Cr,Al) martensite, retained gamma-Fe, and the eutectic comprised of CrFeB, M-3(C,B)(2), M-2(B,C) and M-23(C,B)(6). The average microhardness of the coating (742.1 HV0.5) was 3.95 times compared with the 2Cr13 steel substrate, and the wear resistance was enhanced by 12.5 times compared with the 2Cr13 steel substrate. The wear mechanisms were dominated by abrasive wear and oxidative wear.