The transmission efficiency, bulk temperature, integrate temperature, and scuffing load of test gears were investigated by a modified FZG rig. These values were measured in four different oil immersion depths with one kind of potential oil that may be used to replace the current synthetic oil in order to find the reasonable lubricant quantity that meets not only the need of transmission performance but also the need of scuffing resistance for high-speed train gears. For the purpose of evaluating the efficiency of the test gears, there were two sensors added to measure the output torque of testing gears and the compensation torque of motor. The minimum lubrication film thickness at the pitch point was gained through an EHL model. The ratio of the film thickness A was used to identify the friction status when scuffing occurred at different oil levels. The results demonstrated that no matter what kind of the immersion depth was, the minimum film thickness on the pitch point was about 0.1 mu m and the ratio of film thickness was about 1.5 when scuffing occurred. According to the relationship of contact pressure and ratio of film thickness at the pitch point, it was found that the immersion depth of pinion should not be less than one module of the gear when the contact pressure of high-speed train gear at pitch point was about 580 MPa, which was the actual pressure at start-up stage.