WS2 films with duplex-layer structure were deposited by magnetron sputtering. The tribological differences of asdeposited, AO-irradiated WS2 films and WS2/P201 hybrid system were mainly investigated for the same film. In comparison with as-deposited WS2 film, AO-irradiated WS2 film exhibits the lower initial friction coefficient and lower wear rate, and longer service longevity. Meanwhile, WS2/P201 hybrid system significantly extended service life further. It is found that the generated WS2 debris act as lubricating agent with different participation states and air isolation in the different lubricating systems affect the tribological property of WS2 film. Eventually, different lubrication mechanisms for AO-irradiated WS2 film and WS2/P201 hybrid system in vacuum were revealed.