Composite C-60-LB films were fabricated by the Langmuir-Blodgett (LB) technique, their microstructures, micro- and macro-tribological properties were investigated using atomic force microscope/ friction force microscope (AFM/FFM). The results showed that in the confined C-60-LB films there were two kinds of structures for the special C-60 assembly: grain diameters of one kind were in the range of 150 -230 nm; the other was smaller than 20 nm. Micro-tribological studies showed that topographical images of tiny C60 aggregates (< 20 nm) were consistent with their frictional ones very well, namely, low friction occurred on tiny C-60 aggregates compared with fatty chains LB monolayer, and 'Micro-rolling effect' was apparent; but for big large ones frictional forces were relatively high and 'ratchet mechanism' was seen apparently. Macro-tribological data proved large C-60 aggregates had wear resistance and load-carrying capacities and anti-wear lives for composite C-60-LB films were prolonged greatly with dispersibility of C-60 improved and its grain diameter reduced. Tiny C-60 aggregates were mainly the lubricating agents. Friction coefficients of composite C-60-LB films gradually reduced with loads increasing having the same friction coefficient- load relations with boundary lubrication films.