The shaft bearing is the key component of water pump and its fatigue failure results in the abnormal operation of the system. However, the failure mechanisms of bearing are still unclear. In this paper, the failure analysis on the engine water pump shaft bearing was carried out by using the measurement of material composition and properties, observation of macro and micro morphologies, and theoretical analysis of fatigue failure. The wear behavior and failure mechanisms were clarified, and the prevention measures was further proposed. The bearing failure reflected from the fracture of bearing cage and serious wear of roller and mandrel. The wear behavior of the mandrel originated from the surface fatigue, and the failure was attributed to the large radial deflection load. Further, the decreased weight of impeller and shaft connector and the increased bearing thickness were recommended to improve the bearing operation.