Wear behaviors of materials in nuclear power stations are very complex, because they are rarely subject to just pure impact or sliding wear. This study uses a self-made impact-sliding wear test rig to study the influences of impact kinetic energy and sliding velocity on the energy absorption, mechanical response, interface deformation, and damage behavior of nuclear-grade 304 stainless steels (304SS). The results show that an increase in impact velocity causes an increase in absorbed energy, impact force, and friction force, while lowing the friction coefficient. The friction force and friction coefficient increase with an increase in the sliding velocity, whereas no obvious change is seen in impact force and absorbed energy. The dominant wear mechanism in test samples were delamination failure and friction oxidation. Furthermore, numerical study was performed to explain the effect of velocity on the mechanical response of 304SS.