In this work, large-scale molecular dynamics (MD) computational simulations were performed in order to explore the sliding contact responses of rough surfaces with hexadecane lubricant and added nanoparticles. Simulation results revealed that the frictional state was dependent on the fluid, nanoparticle, and surface roughness. Three lubricating conditions were compared based on considerations of different amounts of fluid molecules. The lubricant was not able to separate the frictional contact surfaces if the quantity of lubricant molecules was insufficient. Particularly, there were no lubricating contributions when the amount of lubricant was too low, and the lubricant therefore only filled the pits in the surface roughness. Thus, the normal load was primarily supported by the contact between the two surfaces and nanoparticles, leading to significant surface morphology changes. In contrast, the frictional contact surfaces were able to be completely separated by the lubricant when there was a sufficient amount of fluid, and a very good lubricating effect could thus be achieved, resulting in a smaller friction force. In addition, the changes in surface morphology, contact area, and RMS are discussed in this paper, in order to reveal the dynamic frictional process.