Abstract Natural gas hydrate is widely distributed, shallow-buried, clean, and pollution-free and has enormous reserves, it is regarded as the alternative clean energy source in the oil and gas field with the most potential. Pressure coring is the only way to drill for gas hydrate core on the surface under in situ conditions, which is of great value for analyzing its occurrence conditions and reserves comprehensively. Based on this, a new wireline pressure coring system (WPCS) with a ball valve seal was designed and developed in this paper; it was applied in the deep sea for the first time in the South China Sea hydrate survey voyage of the Guangzhou Marine Geological Survey (GMGS). A total of 15 runs of deep-sea gas hydrate drilling and coring applications were carried out, and they tested well. The experimental water depth was 1700–1800 m, and the coring depth below the seafloor was about 100–150 m. The formation consisted of sandy hydrate and argillaceous hydrate. The results showed the following. (1) The success rate of ball valve turn-over could reach almost 100% in the argillaceous hydrate reservoir, although there are some isolated cases of pressure relief. Meanwhile, drilling in the sandy hydrate reservoir, the success rate was only 54.55%. (2) When drilling in the argillaceous hydrate reservoir, the core recovery rate could reach 80%, while in the sandy hydrate reservoir, it was almost 0%. In practice, the sandy formation with gas hydrate is stiff to drill compared to the performance in argillaceous formations. After our analysis, it was believed that the ball valve and core tube could be easily plugged by sand debris during the sampling of sandy hydrate formation. Moreover, the sandy core is easily plugged into the core liner because of the high friction of sand grains in clearance. (3) The pressure-holding effect of the core drilling tool was related to the formation of hydrate, the sealing form of the ball valve, and the environmental pressure. Sandy hydrate formations often caused the ball valve to jam, while the muddy hydrate formation did not. The research results of this paper have reference value for the further optimization of the WPCS structure, the optimization of drilling parameters, and the design parameters of the ball valve structure, which could be better used for the pressure coring of gas hydrate and subsequent research work in the future. Keywords: natural gas hydrate; pressure coring; structure design; ball valve seal; core recovery rate